Scientist IV

About This Role

Design, develop, and execute functional genomics assays, including CRISPR-based editing and advanced sequencing technologies. Provide technical guidance and training on these technologies and manage detailed laboratory records and equipment.

Responsibilities

• Design, develop, and execute functional genomics assays, including CRISPR-based editing (e.g., RNP delivery, Perturb-seq), single-cell sequencing, spatial transcriptomics, and CITE-seq
• Provide technical guidance and training to research scientists on FGSL technologies and techniques, including assay execution, troubleshooting, and resolution of technical issues
• Document and maintain detailed laboratory records in accordance with policies
• Operate and maintain lab equipment in collaboration with other lab members
• Assist acquisition of necessary supplies and equipment
• Define and perform appropriate QC measures
• Follow relevant scientific literature to ensure use of optimal methods and understand emerging practices across the field
• Interpret the outcome of experiments, propose appropriate follow-up, and may propose new avenues of investigation
• Communicate own work effectively orally and in writing; contribute to writing protocols, procedures, and technical reports
• Automate processing and results reporting and delivery
• Report and treat data with a high level of integrity and ethics

Requirements

• PhD or MS Degree with 6+ years of experience in molecular or cell biology
• 2 years of CRISPR experience
• CRISPR assays (personally designed guides, performed transfections/infections, selected edited cells, and validated knockouts/knockins)
• Next-generation sequencing (NGS)- hands-on library preparation experience
• Single cell technologies - personal experience with sample prep, library generation, and quality control
• Standard molecular biology techniques (qPCR, Western blot, cloning, gel electrophoresis)
• Proficient in maintaining multiple cell lines, transfections, and sterile technique

Qualifications

• Ph.D. or Master’s degree from an accredited institution in molecular, cell biology, or related scientific discipline
• 6+ years of experience in molecular, cell biology, or related scientific discipline, with 2 years of CRISPR experience

Nice to Have

• Experience in automation

Skills

* Required skills

Benefits