Scientist II – Antibody Development

About This Role

Support the research and development of monoclonal antibodies within the Antibody Technologies team. This role involves protein expression and purification, tissue culture and cell-line engineering, and antibody testing for flow cytometry applications.

Responsibilities

• Purify antibodies and recombinant proteins using AKTA FPLC and manual purification methods
• Execute and document work in accordance with SOPs while adhering to strict GMP requirements under ISO 13485 standards
• Coordinate preparation and transfer of purified antibody and protein samples to other functional teams
• Perform analytical testing of purified antibodies and proteins using SEC, SDS-PAGE, Western blot, MADLS, qPCR
• Perform transfection and/or expression of antibodies and recombinant proteins from various systems, including Mammalian cell lines (e.g., CHO, hybridomas) and E. coli
• Apply advanced molecular biology techniques to execute experiments of varying complexity
• Execute experiments using multi-color flow cytometry
• Analyze flow cytometry data using FCS Express and FlowJo; report results and complete documentation and database entries
• Perform day-to-day problem solving and participate in cross-functional team activities to ensure operational timelines are met
• Independently analyze research data, troubleshoot with minimal guidance, and plan follow-up experiments

Requirements

• Protein purification using liquid chromatography and quality assurance through multiple analytical methods
• Protein expression using various systems, including mammalian cells, insect cells, and bacterial systems
• Proficiency with aseptic technique and cell culture techniques
• Strong analytical and problem-solving skills
• AKTA FPLC
• Protein purification experience
• Protein expression experience
• Molecular biology experience
• Tissue culture experience
• Proficiency in tissue culture techniques, including transfection for protein expression

Qualifications

• Bachelor’s or Master’s degree in Biochemistry, Cellular Biology, Molecular Biology, Immunology, or a related discipline
• 2-4 years of laboratory experience

Nice to Have

• Familiarity with assay automation and automated liquid handlers
• Experience with cell line engineering
• Hands-on experience with flow cytometry and flow data analysis
• Antibody development experience
• Immunology background

Skills

* Required skills