Research Associate / Senior Research Associate – Protein Expression & Analytics

About This Role

This Research Associate / Senior Research Associate position involves supporting protein expression and characterization for a seed-stage venture-backed company developing protein-drug conjugate therapies for metabolic diseases. The role primarily focuses on mammalian cell expression systems using transient transfection and contributes to routine analytical and quality control workflows.

Responsibilities

• Perform transient transfections in CHO and HEK293 cells for recombinant protein expression
• Maintain mammalian cell cultures using aseptic technique, including passaging, expansion, and harvest
• Operate and maintain cell counters (e.g., Vi-CELL or equivalent) for viability and cell density measurements
• Execute routine QC and analytical assays, including SDS-PAGE, SEC-HPLC, and RP-HPLC
• Accurately document experimental procedures, results, and deviations in lab notebooks or ELN
• Support troubleshooting of expression, purification, or analytical issues
• Collaborate closely with scientists across protein purification, bioconjugation and analytical teams
• Assist with general lab operations as needed (inventory, equipment upkeep, method optimization)

Requirements

• Bachelor’s or Master’s degree in Biology, Biochemistry, Biotechnology, or a related field
• 1-4 years of hands-on experience in a biotech or academic lab setting
• Practical experience with mammalian cell culture (CHO and/or HEK293)
• Practical experience with transient transfection workflows
• Practical experience with aseptic technique
• Familiarity with protein QC techniques such as SDS-PAGE and chromatography-based assays
• Familiarity with Microsoft Office programs: Excel and PowerPoint
• Strong organizational skills and attention to detail
• Ability to work independently while contributing effectively to a team

Qualifications

• Bachelor’s or Master’s degree in Biology, Biochemistry, Biotechnology, or a related field
• 1-4 years of hands-on experience in a biotech or academic lab setting

Nice to Have

• Experience with SEC-HPLC and RP-HPLC method execution or data interpretation
• Exposure to protein purification workflows (e.g., affinity chromatography)
• Experience working in a startup or fast-moving research environment
• Comfort learning new techniques and taking on responsibilities outside a narrowly defined role

Skills

* Required skills